Sixties Bunch in Bristol

Sixties Bunch in Bristol

Aleida Spielvog… 0 12 01.06 14:19
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Lung-specific induction of MDM2 expression in mice leads to expression of interphase markers however not DNA replication. To find out the consequence of elevated MDM2 expression in the lungs of adult mice, pTREtightMDM2 (a plasmid expressing MDM2 from CMV promoter controlled by tetracycline response factor) transgenic mouse strains were generated (Figure 1A). The transgenic mouse line tightMDM2 was then generated by crossbreeding pTREtightMDM2 mice with CCSPrtTA transgenic mice (31), capable of expressing a reverse transactivator, rtTA, from CCSP promoter in response to Dox therapy. When treated with Dox, tightMDM2 mouse traces categorical the transactivator rtTA specifically in CCSP-expressing lung cells and activates TRE to induce MDM2 expression. CCSPrtTA (referred in the paper as rtTA) mice, which lacks mdm2 transgene, was used as management. Dox-induced MDM2 expression was confirmed at RNA (Figure 1B) and protein levels (Figure 1C) by quantitative PCR (qPCR) and immunoblot evaluation, respectively. Dox therapy for three months resulted in an roughly 8-fold enhance in MDM2 transcript levels and a 2-fold increase in the protein ranges in lung.

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